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Identification and characterization of a novel alkalistable and salt-tolerant esterase from the deep-sea hydrothermal vent of the East Pacific Rise
| Content Provider | Scilit |
|---|---|
| Author | Yang, Xinwei Wu, Lianzuan Xu, Ying Ke, Chongrong Hu, Fangfang Xiao, Xiang Huang, Jianzhong |
| Copyright Year | 2018 |
| Description | Journal: MicrobiologyOpen A novel esterase gene selected from metagenomic sequences of deep-sea hydrothermal vents was successfully expressed in Escherichia coli. The recombinant protein (est-OKK), which belongs to the lipolytic enzyme family V, exhibited high activity toward pNP-esters with short acyl chains and especially p-nitrophenyl butyrate. Site-mutagenesis results confirmed that est-OKK contains the nonclassical catalytic tetrad predicted by alignment and computational modeling. The est-OKK protein is a moderately thermophilic enzyme that is relatively thermostable, and highly salt-tolerant, which remained stable in 3 mol/L NaCl for 6 hr. The est-OKK protein showed the considerable alkalistability, displayed optimal activity at pH 9.0 and maintained approximately 70% of its residual activity after incubation at pH 10 for 4 hr. Furthermore, the est-OKK activity was strongly resistant to a variety of metal ions such as $Co^{2+}$, $Zn^{2+}$, $Fe^{2+}$, $Na^{+}$, and $K^{+}$; nonionic detergents such as Tween-20, Tween-80; and organic solvents such as acetone and isopropanol. Taken together, the novel esterase with unique characteristics may give us a new insight into the family V of lipolytic enzymes, and could be a highly valuable candidate for biotechnological applications such as organic synthesis reactions or food and pharmaceutical industries. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182558/pdf |
| ISSN | 20458827 |
| e-ISSN | 20458827 |
| DOI | 10.1002/mbo3.601 |
| Journal | MicrobiologyOpen |
| Issue Number | 5 |
| Volume Number | 7 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2018-03-05 |
| Access Restriction | Open |
| Subject Keyword | Journal: MicrobiologyOpen Biotechnology and Microbiology Deep‐sea Hydrothermal Vents Metagenomic Sequence‐based Strategy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
