NDLI: Integration of diffraction phase microscopy and Raman imaging for label‐free morpho‐molecular assessment of live cells

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Integration of diffraction phase microscopy and Raman imaging for label‐free morpho‐molecular assessment of live cells

Content Provider	Scilit
Author	Pandey, Rishikesh Zhou, Renjie Bordett, Rosalie Hunter, Ciera Glunde, Kristine Barman, Ishan Valdez, Tulio Finck, Christine
Copyright Year	2018
Description	Journal: Journal of Biophotonics Label-free quantitative imaging is highly desirable for studying live cells by extracting pathophysiological information without perturbing cell functions. Here, we demonstrate a novel label-free multimodal optical imaging system with the capability of providing comprehensive morphological and molecular attributes of live cells. Our morpho-molecular microscopy (3M) system draws on the combined strength of quantitative phase microscopy (QPM) and Raman microscopy to probe the morphological features and molecular fingerprinting characteristics of each cell under observation. While the commonr-path geometry of our QPM system allows for highly sensitive phase measurement, the Raman microscopy is equipped with dual excitation wavelengths and utilizes the same detection and dispersion system, making it a distinctive multi-wavelength system with a small footprint. We demonstrate the applicability of the 3M system by investigating nucleated and nonnucleated cells. This integrated label-free platform has a promising potential in preclinical research, as well as in clinical diagnosis in the near future.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447451/pdf
e-ISSN	18640648
DOI	10.1002/jbio.201800291
Journal	Journal of Biophotonics
Issue Number	4
Volume Number	12
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2018-12-13
Access Restriction	Open
Subject Keyword	Journal: Journal of Biophotonics Biotechnology and Microbiology Microscopic Research Raman Spectroscopy Cellular Imaging Label-free Imaging Quantitative Phase Microscopy
Content Type	Text
Resource Type	Article

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