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Molecular and functional characterization of vacuolar‐ATPasefrom theAmerican dog tickDermacentor variabilis
| Content Provider | Scilit |
|---|---|
| Author | Petchampai, N. Sunyakumthorn, P. Guillotte, M. L. Thepparit, C. Kearney, M. T. Mulenga, A. Azad, A. F. Macaluso, K. R. |
| Copyright Year | 2013 |
| Description | Journal: Insect Molecular Biology Vacuolar (V)-ATPase is a proton-translocating enzyme that acidifies cellular compartments for various functions such as receptor-mediated endocytosis, intracellular trafficking and protein degradation. Previous studies in Dermacentor variabilis chronically infected with Rickettsia montanensis have identified V-ATPase as one of the tick-derived molecules transcribed in response to rickettsial infection. To examine the role of the tick V-ATPase in tick–Rickettsia interactions, a full-length 2887-bp cDNA (2532-bp open reading frame) clone corresponding to the transcript of the V0 domain subunit a of D. variabilis V-ATPase (DvVATPaseV0a) gene encoding an 843 amino acid protein with an estimated molecular weight of ∼96 kDa was isolated from D. variabilis. Amino acid sequence analysis of DvVATPaseV0a showed the highest similarity to VATPaseV0a from Ixodes scapularis. A potential N-glycosylation site and eight putative transmembrane segments were identified in the sequence. Western blot analysis of tick tissues probed with polyclonal antibody raised against recombinant DvVATPaseV0a revealed the expression of V-ATPase in the tick ovary. Transcriptional profiles of DvVATPaseV0a demonstrated a greater mRNA expression in the tick ovary, compared with the midgut and salivary glands; however, the mRNA level in each of these tick tissues remained unchanged after infection with R. montanensis for 1 h. V-ATPase inhibition bioassays resulted in a significant decrease in the ability of R. montanensis to invade tick cells in vitro, suggesting a role of V-ATPase in rickettsial infection of tick cells. Characterization of tick-derived molecules involved in rickettsial infection is essential for a thorough understanding of rickettsial transmission within tick populations and the ecology of tick-borne rickettsial diseases. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237192/pdf |
| Ending Page | 51 |
| Page Count | 10 |
| Starting Page | 42 |
| e-ISSN | 13652583 |
| DOI | 10.1111/imb.12059 |
| Journal | Insect Molecular Biology |
| Issue Number | 1 |
| Volume Number | 23 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2013-10-25 |
| Access Restriction | Open |
| Subject Keyword | Journal: Insect Molecular Biology Dermacentor Variabilis Rickettsia Montanensis |
| Content Type | Text |
| Resource Type | Article |
