NDLI: The primary structure of a protein containing a putative [6Fe-6S] prismane cluster from Desulfovibrio vulgaris (Hildenborough)

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The primary structure of a protein containing a putative [6Fe-6S] prismane cluster from Desulfovibrio vulgaris (Hildenborough)

Content Provider	Scilit
Author	Stokkermans, Jack P. W. G. Pierik, Antonio J. Wolbert, Ronnie B. G. Hagen, Wilfred R. Dongen, Walter M. A. M. Veeger, Cees
Copyright Year	1992
Description	Journal: Journal of Biological Inorganic Chemistry The gene encoding a protein containing a putative [6Fe-6S] prismane cluster has been cloned from Desulfovibrio vulgaris (Hildenborough) and sequenced. The gene encodes a polypeptide composed of 553 amino acids (60,161 Da). The DNA-derived amino acid sequence was partly confirmed by N-terminal sequencing of the purified protein and of fragments of the protein generated by CNBr cleavage. Furthermore, the C-terminal sequence was verified by digestion with carboxypeptidases A and B. The polypeptide contains nine Cys residues. Four of these residues are gathered in a Cys-Xaa2-Cys-Xaa7-Cys-Xaa5-Cys motif located towards the N-terminus of the protein. No relevant sequence similarity was found with other proteins, including those with high-spin Fe-S clusters (nitrogenase, hydrogenase), with one significant exception: the stretch containing the first four Cys residues spans two submotifs, Cys-Xaa2-Cys and Lys-Gly-Xaa-Cys-Gly, separated by 11 residues, that are also present in high-spin Fe-S cluster containing CO dehydrogenase. Western-blot analysis demonstrates cross-reactivity of antibodies raised against the purified protein both in Desulfovibrio strains and other sulfate-reducing bacteria. Hybridization of the cloned gene with genomic DNA of several other Desulfovibrio species indicates that homologous sequences are generally present in the genus Desulfovibrio.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1992.tb17205.x/pdf
Ending Page	442
Page Count	8
Starting Page	435
ISSN	09498257
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1992.tb17205.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	2
Volume Number	208
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1992-09-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Biotechnology and Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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