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Comparison of the Properties of Semipurified Mitochondrial and Cytosolic Monoamine Oxidases from Rat Brain
| Content Provider | Scilit |
|---|---|
| Author | Mayanil, C. S. K. Baquer, N. Z. |
| Copyright Year | 1984 |
| Description | Journal: Journal of Neurochemistry Mitochondrial and cytosolic monoamine oxidases were purified 220- and 129-fold, respectively, from rat brain. The purification procedure involved extraction (without the use of detergents for mitochondrial monoamine oxidase), ammonium sulfate precipitation, and chromatography on Sephadex G-25 and a DEAE-cellulose column. The properties of both enzymes with kynuramine as substrate, including Km values and pH optima at different kynuramine concentrations; the Rf values on polyacrylamide gel electrophoresis; and the thermal inactivation patterns were different. 2-Mercaptoethanol, together with heat treatment, released the flavin and decreased the enzyme activity differentially for the two enzymes. The absorption spectrum showed a "Red shift" in the absorption maxima when the spectra of the non-Triton-treated purified preparations were compared with those of the Triton-treated ones, thus possibly revealing that the mitochondrial and the cytosolic monoamine oxidases may be two different enzyme entities. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1984.tb12824.x/pdf |
| Ending Page | 912 |
| Page Count | 7 |
| Starting Page | 906 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1984.tb12824.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 4 |
| Volume Number | 43 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1984-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Monoamine Oxidase Mitochondrial Monoamine Oxidase Cytosolic Monoamine Oxidase |
| Content Type | Text |
| Resource Type | Article |
