Multireceptor analysis in human neocortex reveals complex alterations of receptor ligand binding in focal epilepsies

Content Provider	Scilit
Author	Palomero-Gallagher, Nicola Schleicher, Axel Bidmon, Hans-J. Pannek, Heinz-W. Hans, Volkmar Gorji, Ali Speckmann, Erwin-J. Zilles, Karl
Copyright Year	2012
Description	Journal: Epilepsia Purpose: A disturbed balance between excitatory and inhibitory neurotransmission underlies epileptic activity, although reports concerning neurotransmitter systems involved remain controversial. Methods: We quantified densities of 15 receptors in neocortical biopsies from patients with pharmacoresistant focal temporal lobe epilepsy and autopsy controls, and searched for correlations between density alterations and clinical factors or the occurrence of spontaneous synaptic potentials in vitro. Key Findings: α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), kainate, N-methyl-d-aspartate (NMDA), peripheral benzodiazepine, muscarinic $(M)_{1}$, $M_{2}$, nicotinic, $α_{1}$, $α_{2h}$, serotonin $(5-HT)_{1A}$, and adenosine $(A)_{1}$ receptor densities were significantly altered in biopsies. The epileptic cohort was subdivided based on clinical (febrile seizures, hippocampal sclerosis, neocortical pathologies, surgery outcome) or electrophysiologic (spontaneous field potentials) criteria, resulting in different patterns of significantly altered receptor types when comparing a given epileptic group with controls. Only AMPA, kainate, $M_{2}$, and $5-HT_{1A}$ receptors were always significantly altered. γ-Aminobutyric acid $(GABA)_{A}$, $GABA_{B}$, and $5-HT_{2}$ receptor alterations were never significant. Correlation patterns between receptor alterations and illness duration or seizure frequency varied depending on whether the epileptic cohort was considered as a whole or subdivided. Significance: Neocortical temporal lobe epilepsy is associated with a generalized receptor imbalance resulting in a net potentiation of excitatory neurotransmission. Peripheral benzodiazepine receptor alterations highlight that astrocytes are also impaired by seizure activity.
Related Links	https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1528-1167.2012.03634.x
Ending Page	1997
Page Count	11
Starting Page	1987
e-ISSN	15281167
DOI	10.1111/j.1528-1167.2012.03634.x
Journal	Epilepsia
Issue Number	11
Volume Number	53
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2012-09-07
Access Restriction	Open
Subject Keyword	Journal: Epilepsia Temporal Lobe Epilepsy Neurotransmitter Receptors
Content Type	Text
Resource Type	Article