Loading...
Please wait, while we are loading the content...
Similar Documents
Functional Regulation of Alternatively Spliced Na+/Ca2+ Exchanger (NCX1) Isoforms
| Content Provider | Scilit |
|---|---|
| Author | Schulze, D. H. Polumuri, S. K. Gille, T. Ruknudin, A. |
| Copyright Year | 2006 |
| Description | Journal: Annals of the New York Academy of Sciences Alternative splicing of RNA transcripts is a general characteristic for NCX genes in mammals, mollusks, and arthropods. Among the family of three NCX genes in mammals, the NCX1 gene contains six exons, namely, A, B, C, D, E, and F, that make up the alternatively spliced region. Studies of the NCX1 gene transcripts suggested that 16 distinct gene products can be produced from the NCX1 gene. The exons A and B are mutually exclusive when expressed. Generally, exon A‐containing transcripts are predominantly found in excitable cells like cardiomyoctes and neurons, whereas exon B‐containing transcripts are mostly found in nonexcitable cells like astrocytes and kidney cells. Other alternatively spliced exons (C‐F) appear to be cassette‐type exons and are found in various combinations. Interestingly, exon D is present in all characterized transcripts. The alternatively spliced isoforms of NCX1 show tissue‐specific expression patterns, suggesting functional adaptation to tissues. To investigate functional differences among alternatively spliced isoforms of NCX1, we expressed an exon A‐containing transcript present in cardiac tissue (NCX1.1) and an exon B‐containing transcript found in the kidney (NCX1.3) in Xenopus oocytes. We demonstrated that the $Na^{+}/Ca^{2+}$ exchangers expressed by exon A‐ and exon B‐containing transcripts display differences in activation by PKA and by [Ca^{2+}$]_{i}$. We also observed that these two isoforms show differences in voltage dependence. Suprisingly, the alternatively spliced isoforms of NCX1 display greater functional differences among themselves than the products of different gene loci, NCX1, NCX2, and NCX3. |
| Related Links | https://nyaspubs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1749-6632.2002.tb04740.x |
| Ending Page | 196 |
| Page Count | 10 |
| Starting Page | 187 |
| e-ISSN | 17496632 |
| DOI | 10.1111/j.1749-6632.2002.tb04740.x |
| Journal | Annals of the New York Academy of Sciences |
| Issue Number | 1 |
| Volume Number | 976 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2006-01-24 |
| Access Restriction | Open |
| Subject Keyword | Journal: Annals of the New York Academy of Sciences Biochemistry and Molecular Biology Sodium‐calcium Exchanger Alternative Splicing |
| Content Type | Text |
| Resource Type | Article |
