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Methylamine-induced conformational change of alpha2-macroglobulin and its zinc(II) binding capacity. An X-ray scattering study
| Content Provider | Scilit |
|---|---|
| Author | Osterberg, Ragnar Malmensten, Bo |
| Copyright Year | 1984 |
| Description | Journal: Journal of Biological Inorganic Chemistry Methylamine induces a conformational change of $α_{2}$‐macroglobulin which is very similar to that obtained by proteinase reaction and binding. This was shown by small‐angle X‐ray scattering at 21 °C in 0.03 M Hepes buffer of pH 8.0 containing 0.15 M NaCl and 0.3 mM EDTA. When $α_{2}$‐macroglobulin reacts with methylamine the side maximum virtually disappears from the X‐ray scattering curve and the radius of gyration decreases from 7.8 nm to 7.2 nm. The X‐ray data of $α_{2}$‐macroglobulin are consistent with an open shape model similar to that deduced via electron micrographs [Schramm, H. J. and Schramm, W. (1982) Hoppe‐Seyler's Z. Physiol. Chem. 363, 803–812]; one projection of the model resembles the letter H; the four subunits are mainly represented as elliptical cylinders which are connected via a central, quite flat cylinder. Zinc(II) ions cause aggregation of $α_{2}$‐macroglobulin even at such a low total zinc concentration as 12.5 μM; for 25 μM zinc(II) concentration, the average molecular mass indicates that the aggregation goes beyond the dimeric stage. Monomeric species of $α_{2}$‐macroglobulin appear to have the capacity specifically to bind 8.0 zinc(II) ions per molecule, which corresponds to two zinc(II) ions per subunit. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1984.tb08403.x/pdf |
| Ending Page | 544 |
| Page Count | 4 |
| Starting Page | 541 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1984.tb08403.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 3 |
| Volume Number | 143 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1984-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Microscopic Research Conformational Change |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
