NDLI: The Binding of Low-Affinity and High-Affinity Heparin to Antithrombin. Fluorescence Studies

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The Binding of Low-Affinity and High-Affinity Heparin to Antithrombin. Fluorescence Studies

Content Provider	Scilit
Author	Nordenman, Birgitta Danielsson, Ake Bjork, Ingemar
Copyright Year	1978
Description	Journal: Journal of Biological Inorganic Chemistry The interaction between antithrombin and two forms of heparin, differing in their affinity for the matrix-linked protein, has been studied by fluorescence. The binding of the high-affinity heparin fraction to antithrombin leads to activation of the inhibitor, allowing it to react more rapidly with a number of serine proteases of the coagulation cascade. The interaction with the low-affinity heparin fraction, however, has considerably less influence on this inhibition rate. The binding of either fraction to antithrombin was found to result in an increase of the tryptophan fluorescence of the protein. This increase was much larger for high-affinity heparin than for low-affinity heparin, suggesting a different mode of binding of the two fractions to the protein. The fluorescence enhancement caused by high-affinity heparin is consistent with a conformational change of antithrombin related to its activation. Only the fluorescence enhancement observed on the binding of high-affinity heparin was of a sufficient magnitude to allow quantitative studies. These showed high-affinity heparin to bind to antithrombin with a stoichiometry of about one and with a binding constant at physiological ionic strength of about 8 X 10(7) M-1. At higher ionic strengths, however, the affinity decreased markedly.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1978.tb12567.x/pdf
Ending Page	6
Page Count	6
Starting Page	1
ISSN	09498257
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1978.tb12567.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	1
Volume Number	90
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1978-09-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Conformational Change High Affinity Heparin
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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