Loading...
Please wait, while we are loading the content...
Similar Documents
A Novel Monoclonal Antibody Specific for Sialylated MUC1 Mucin
| Content Provider | Scilit |
|---|---|
| Author | Yamamoto, Masaya Bhavanandan, V. P. Nakamori, Shoji Irimura, Tatsuro |
| Copyright Year | 1996 |
| Description | Journal: Japanese Journal of Cancer Research Development of a new monoclonal antibody (mAb) MY.1E12 which reacts with sialylated MUC1 mucins is described. The mAb did not react with any component in the lysates of COS‐1 cells, whereas it bound to sialylated MUC1 mucins produced by COS‐1 cells transiently transacted with MUC1 mucin cDNA, strongly suggesting that the expression of the epitope of mAb MY.1E12 depends on the presence of the MUC1 mucin core peptide. The requirement of sialyl residues for antibody recognition was established by Western blotting analysis of extracts of various carcinoma cells and in situ desialylation. In all cases, the mAb binding of electrophoretically separated MUC1 mucin diminished after desialylation by mild acid hydrolysis. When Capan‐1 pancreatic carcinoma cells were pretreated with benzyl‐JV‐acetylgalactosaminide in culture, the MUC1 mucins produced under these conditions, which were detected by core peptide‐specific mAbs, did not react with mAb MY.1E12. These results suggest that 0‐linked carbohydrate chains are important for the mAb binding. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921115/pdf http://onlinelibrary.wiley.com/doi/10.1111/j.1349-7006.1996.tb00250.x/pdf |
| Ending Page | 496 |
| Page Count | 9 |
| Starting Page | 488 |
| ISSN | 09105050 |
| DOI | 10.1111/j.1349-7006.1996.tb00250.x |
| Journal | Japanese Journal of Cancer Research |
| Issue Number | 5 |
| Volume Number | 87 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1996-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Japanese Journal of Cancer Research Carcinoma‐associated Epitope Monoclonal Antibody |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
