Loading...
Please wait, while we are loading the content...
Similar Documents
Localization of Lys-51 of L7/L12 on 50S ribosomes from Escherichia coli
| Content Provider | Scilit |
|---|---|
| Author | Maassen, J. Antonie Schop, Enno N. Moller, Wim |
| Copyright Year | 1984 |
| Description | Journal: Journal of Biological Inorganic Chemistry Ribosomal protein L7/L12 from Escherichia coli was modified specifically at Lys-51 with 4-(6-formyl-3-azido-phenoxy)butyrimidate. Reconstitution of ribosomal cores, lacking L7/L12, with imidate-modified L7/L12 resulted in back formation of 50S particles which were fully active in elongation-factor-dependent processes. By use of the formylazidophenoxy moiety as hapten, the position of Lys-51 of L7/L12 on the 50S ribosome was determined by immune electron microscopy. The results show that an L7/L12 dimer is present in the L7/L12 stalk in such a way that Lys-51 is located at the far cytoplasmic end of the stalk. The experimental data are discussed in relation to a proposed model for the L7/L12 dimer. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1984.tb07891.x/pdf |
| Ending Page | 134 |
| Page Count | 4 |
| Starting Page | 131 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1984.tb07891.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 138 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1984-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Escherichia Coli 50s Ribosome L7/l12 Dimer |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
