NDLI: Transcription and genetic analyses of a putative N-acetylmuramyl-l-alanine amidase in Borrelia burgdorferi

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Transcription and genetic analyses of a putative N-acetylmuramyl-l-alanine amidase in Borrelia burgdorferi

Content Provider	Scilit
Author	Yang, Yu Li, Chunhao
Copyright Year	2008
Description	Journal: FEMS Microbiology Letters In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb0665 and bb0667) are cotranscribed with the motility and chemotaxis genes. This newly identified operon is termed as pami. Sequence and primer extension analyses showed that pami was regulated by a $σ^{70}$-like promoter, which is designated as $P_{ami}$. Transcriptional analysis using a gene encoding green fluorescence protein as a reporter demonstrated that $P_{ami}$ functions in both Escherichia coli and B. burgdorferi. Genetic studies showed that the Δbb0666 mutant grows in long chains of unseparated cells, whose phenotype is similar to its counterparts in E. coli. Taken together, these results demonstrate that bb0666 is a homolog of MurNac-LAAs that contributes to the cell division of B. burgdorferi.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927206/pdf
Ending Page	173
Page Count	10
Starting Page	164
ISSN	03781097
e-ISSN	15746968
DOI	10.1111/j.1574-6968.2008.01416.x
Journal	FEMS Microbiology Letters
Issue Number	2
Volume Number	290
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2008-12-08
Access Restriction	Open
Subject Keyword	Journal: FEMS Microbiology Letters Biochemistry and Molecular Biology Lyme Disease Borrelia Burgdorferi N-acetylmuramyl-l-alanine Amidase
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Microbiology

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