pkc-1 regulates daf-2 insulin/IGF signalling-dependent control of dauer formation in Caenorhabditis elegans

Content Provider	Scilit
Author	Monje, José M. Brokate-Llanos, Ana M. Pérez-Jiménez, Mercedes M. Fidalgo, Manuel A. Muñoz, Manuel J.
Copyright Year	2011
Description	Journal: Aging Cell In Caenorhabditis elegans, the insulin/IGF pathway participates in the decision to initiate dauer development. Dauer is a diapause stage that is triggered by environmental stresses, such as a lack of nutrients. Insulin/IGF receptor mutants arrest constitutively in dauer, an effect that can be suppressed by mutations in other elements of the insulin/IGF pathway or by a reduction in the activity of the nuclear hormone receptor daf-12. We have isolated a pkc-1 mutant that acts as a novel suppressor of the dauer phenotypes caused by insulin/IGF receptor mutations. Interactions between insulin/IGF mutants and the pkc-1 suppressor mutant are similar to those described for daf-12 or the DAF-12 coregulator din-1. Moreover, we show that the expression of the DAF-12 target daf-9, which is normally elevated upon a reduction in insulin/IGF receptor activity, is suppressed in a pkc-1 mutant background, suggesting that pkc-1 could link the daf-12 and insulin/IGF pathways. pkc-1 has been implicated in the regulation of peptide neurosecretion in C. elegans. Although we demonstrate that pkc-1 expression in the nervous system regulates dauer formation, our results suggest that the requirement for pkc-1 in neurosecretion is independent of its role in modulating insulin/IGF signalling. pkc-1 belongs to the novel protein kinase C (nPKC) family, members of which have been implicated in insulin resistance and diabetes in mammals, suggesting a conserved role for pkc-1 in the regulation of the insulin/IGF pathway.This work was supported by the Spanish Ministry of Science (MICINN) BF -07391 ⁄ BMC, the European Regional Development Fund (FEDER) and the Junta de Andalucía Project P07-CVI-02697. JMM was supported by the FPU program of the Spanish Ministry of Science. AMB was supported by a Plan Propio de Investigación fellowship from UPO. MMP was supported by a Junta de Andalucía fellowship.Peer Reviewe
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1474-9726.2011.00747.x/pdf
Ending Page	1031
Page Count	11
Starting Page	1021
e-ISSN	14749726
DOI	10.1111/j.1474-9726.2011.00747.x
Journal	Aging Cell
Issue Number	6
Volume Number	10
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2011-11-14
Access Restriction	Open
Subject Keyword	Journal: Aging Cell Nuclear Hormone Protein Kinase C C. Elegans
Content Type	Text
Resource Type	Article