NDLI: Purification and Properties of a DNA-Dependent ATPase Induced by Bacteriophage T4

Please wait, while we are loading the content...

Purification and Properties of a DNA-Dependent ATPase Induced by Bacteriophage T4

Content Provider	Scilit
Author	Purkey, Robert M. Ebisuzaki, Kaney
Copyright Year	1977
Description	Journal: Journal of Biological Inorganic Chemistry A DNA-dependent ATPase formed after T4 phage infection is purified to apparent homogeneity. The molecular weight of the purified enzyme is 50 000 when determined by glycerol gradient centrifugation and by sodium dodecylsulfate/polyacrylamide gel electrophoresis. The enzyme at an earlier stage in purification (prior to DEAE-cellulose chromatography) exists as a complex with a molecular weight of 100000. However, molecular weight determinations by Sephadex gel chromatography give considerably decreased molecular weights for the complex and for the enzyme after DEAE-cellulose chromatography. The enzyme is stimulated to varying degrees by a variety of single-stranded polydeoxyribonucleotides or by single-stranded DNA, but no chemical change in the polynucleotide has been detected as a result of the enzyme action.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1977.tb11530.x/pdf
Ending Page	310
Page Count	8
Starting Page	303
ISSN	09498257
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1977.tb11530.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	1
Volume Number	75
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1977-05-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in