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Kinetic Properties of Hxdrogenase Isolated from Desulfovibrio vulgaris (Hildenborough)
| Content Provider | Scilit |
|---|---|
| Author | Grande, Hans J. Berkel-Arts, Anita Bregh, Jan Dijk, Kees Veeger, Cees |
| Copyright Year | 1983 |
| Description | Journal: Journal of Biological Inorganic Chemistry Hydrogenase of Desulfovibrio vulgaris shows nonlinear kinetics in hydrogen production with both the natural electron carrier, cytochrome c3, and the artificial donor, methyl viologen semiquinone. Increasing concentrations of salt progressively inhibit the hydrogen production, as do increasing amounts of dimethylsulfoxide (Me2SO). Hydrogen consumption activity does not change up to 30% (v/v) of Me2SO. Preincubation in Me2SO up to 55% (v/v) does not affect the hydrogen uptake or production. The production activity of the enzyme shows an optimum around pH 6. When plotted as a function of redox potential the activity can be fitted to a Nernst equation with n = 1. Midpoint potentials calculated at various values follow approximately the hydrogen electrode to pH 6. Thereafter, there is a shift of about 40 mV to higher redox potentials. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1983.tb07233.x/pdf |
| Ending Page | 88 |
| Page Count | 8 |
| Starting Page | 81 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1983.tb07233.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 131 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1983-03-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Water Science and Technology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
