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Profiling the metabolome changes caused by cranberry procyanidins in plasma of female rats $using^{1}$H NMR and UHPLC-Q-Orbitrap-HRMS global metabolomics approaches
| Content Provider | Scilit |
|---|---|
| Author | Liu, Haiyan Garrett, Timothy J. Tayyari, Fariba Gu, Liwei |
| Copyright Year | 2015 |
| Description | Journal: Molecular Nutrition & Food Research The objective was to investigate the metabolome changes in female rats gavaged with partially purified cranberry procyanidins (PPCP) using (1) H NMR and UHPLC-Q-Orbitrap-HRMS metabolomics approaches, and to identify the contributing metabolites. Twenty-four female Sprague-Dawley rats were randomly separated into two groups and administered PPCP or partially purified apple procyanidins (PPAP) for three times using a 250 mg extracts/kg body weight dose. Plasma was collected 6 h after the last gavage and analyzed using (1) H NMR and UHPLC-Q-Orbitrap-HRMS. No metabolome difference was observed using (1) H NMR metabolomics approach. However, LC-HRMS metabolomics data show that metabolome in the plasma of female rats administered PPCP differed from those gavaged with PPAP. Eleven metabolites were tentatively identified from a total of 36 discriminant metabolic features based on accurate masses and/or product ion spectra. PPCP caused a greater increase of exogenous metabolites including p-hydroxybenzoic acid, phenol, phenol-sulphate, catechol sulphate, 3, 4-dihydroxyphenylvaleric acid, and 4'-O-methyl-(-)-epicatechin-3'-O-beta-glucuronide in rat plasma. Furthermore, the plasma level of O-methyl-(-)-epicatechin-O-glucuronide, 4-hydroxy-5-(hydroxyphenyl)-valeric acid-O-sulphate, 5-(hydroxyphenyl)-ϒ-valerolactone-O-sulphate, 4-hydroxydiphenylamine, and peonidin-3-O-hexose were higher in female rats administered with PPAP. The metabolome changes caused by cranberry procyanidins were revealed using an UHPLC-Q-Orbitrap-HRMS global metabolomics approach. Exogenous and microbial metabolites were the major identified discriminate biomarkers. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4715629/pdf |
| Ending Page | 2118 |
| Page Count | 12 |
| Starting Page | 2107 |
| e-ISSN | 16134133 |
| DOI | 10.1002/mnfr.201500236 |
| Journal | Molecular Nutrition & Food Research |
| Issue Number | 11 |
| Volume Number | 59 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2015-09-15 |
| Access Restriction | Open |
| Subject Keyword | Journal: Molecular Nutrition & Food Research Agriculture and Animal Production Uhplc-q-orbitrap-hrms |
| Content Type | Text |
| Resource Type | Article |
