Loading...
Please wait, while we are loading the content...
Similar Documents
Local Complement C3 Expression is Upregulated in Humoral and Cellular Rejection of Renal Allografts
| Content Provider | Scilit |
|---|---|
| Author | Serinsöz, Ebru Bock, Oliver Gwinner, Wilfried Schwarz, Anke Haller, Hermann Kreipe, Hans Mengel, Michael |
| Copyright Year | 2005 |
| Description | Journal: American Journal of Transplantation Evidence on the role of the complement system in transplantation pathology has been accelerated by the discovery of C4d as an in situ marker of antibody-mediated rejection. However, a local or systemic source of complement expression during acute rejection is under discussion. Thus, we quantitatively analyzed local RNA expression of complement component C3 as a pivotal molecule in active humoral and cellular rejection of renal allografts. After laser microdissection, real-time RT-PCR was performed for C3 using RNA extracted from tubuli and glomeruli of 68 paraffin-embedded renal allograft biopsies. Protocol and indication biopsies with signs of humoral and/or cellular rejection were investigated. Quantitative expression analysis of cytokines (IFN gamma, MCP-1, IL2, IL8) potentially influencing local C3 expression was performed. We observed a significant increase in median expression level of C3 mRNA in tubuli of C4d-positive indication biopsies, and in tubuli from indication biopsies with signs of T-cell-mediated cellular rejection. Highest expression levels were found in C4d-positive indication biopsies with signs of cellular rejection. Biopsies with upregulated C3 showed increased IFN gamma expression, suggesting allograft-infiltrating T-cells as potential stimulus for local C3 expression. Therefore, locally synthesized complement component C3 contributes to both humoral and cellular rejection, with tubular epithelial cells being a major source. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1600-6143.2005.00873.x/pdf |
| Ending Page | 1494 |
| Page Count | 5 |
| Starting Page | 1490 |
| e-ISSN | 16006143 |
| DOI | 10.1111/j.1600-6143.2005.00873.x |
| Journal | American Journal of Transplantation |
| Issue Number | 6 |
| Volume Number | 5 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2005-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: American Journal of Transplantation Urology and Nephrology Renal Transplantation |
| Content Type | Text |
| Resource Type | Article |
