NDLI: Suppressive Effect of Liposomes Containing DNA Coding for Diphtheria Toxin A-Chain on Cells Transformed with Bovine Leukemia Virus

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Suppressive Effect of Liposomes Containing DNA Coding for Diphtheria Toxin A-Chain on Cells Transformed with Bovine Leukemia Virus

Content Provider	Scilit
Author	Kakidani, Hitoshi Watarai, Shinobu Onuma, Misao Tomochika, Ken-Ichi Yasuda, Tatsuji
Copyright Year	1993
Description	Journal: Microbiology and Immunology A recombinant plasmid which contained a gene for diphtheria toxin A-chain (DT-A) under the control of the long terminal repeat (LTR) of bovine leukemia virus (BLV) (BLV-LTR) was constructed to test a novel application of liposomes as antiviral agents. The promoter activity of BLV-LTR was estimated by the chloramphenicol acetyltransferase (CAT) assay using a plasmid which contains the coding sequence of CAT under the control of BLV-LTR (pBLVCAT). When BLV-infected cells were transfected with pBLVCAT, CAT activity was detected. BLV-uninfected cell lines, however, showed no detectable CAT activity. The plasmid DNA entrapped in liposomes was added to BLV-infected cells in culture. Syncytium formation induced by BLV-infected cells was effectively suppressed by the liposomes containing the gene for DT-A under the control of BLV-LTR. Conversely, liposomes containing the gene for DT-A without a promoter showed no such effect. DT-A gene-containing liposomes with BLV-LTR did not affect formation of syncytium induced by bovine immunodeficiency virus. These observations indicate that BLV-infected cells were readily targeted on the level of gene expression. This strategy could be applied to the treatment of BLV-induced B-cell proliferation of cattle, and further to other viral/neoplastic diseases where specific gene expression is exerted.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1348-0421.1993.tb01696.x/pdf
Ending Page	720
Page Count	8
Starting Page	713
ISSN	03855600
e-ISSN	13480421
DOI	10.1111/j.1348-0421.1993.tb01696.x
Journal	Microbiology and Immunology
Issue Number	9
Volume Number	37
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1993-09-01
Access Restriction	Open
Subject Keyword	Journal: Microbiology and Immunology Pharmacology and Pharmacy Bovine Leukemia Virus Diphtheria Toxin Gene Therapy
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology

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