NDLI: Crystal structure of exo‐rhamnogalacturonan lyase fromPenicillium chrysogenumas a member of polysaccharide lyase family 26

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Crystal structure of exo‐rhamnogalacturonan lyase fromPenicillium chrysogenumas a member of polysaccharide lyase family 26

Content Provider	Scilit
Author	Kunishige, Yuika Iwai, Marin Nakazawa, Masami Ueda, Mitsuhiro Tada, Toshiji Nishimura, Shigenori Sakamoto, Tatsuji
Copyright Year	2017
Description	Journal: FEBS Letters Exo‐rhamnogalacturonan lyase from Penicillium chrysogenum 31B (PcRGLX) was recently classified as a member of polysaccharide lyase (PL) family 26 along with hypothetical proteins derived from various organisms. In this study, we determined the crystal structure of PcRGLX as the first structure of a member of this family. Based on the substrate‐binding orientation and substrate specificity, PcRGLX is an exo‐type PL that cleaves rhamnogalacturonan from the reducing end. Analysis of PcRGLX‐complex structures with reaction products indicate that the active site possesses an L‐shaped cleft that can accommodate galactosyl side chains, suggesting side‐chain‐bypassing activity in PcRGLX. Furthermore, we determined the residues critical for catalysis by analyzing the enzyme activities of inactive variants.
Ending Page	1388
Starting Page	1378
ISSN	2573508X
e-ISSN	18733468
DOI	10.1002/1873-3468.13034
Journal	FEBS Letters
Issue Number	8
Volume Number	592
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2017-12-29
Access Restriction	Open
Subject Keyword	Journal: FEBS Letters Biochemistry and Molecular Biology Biotechnology and Microbiology Crystal Structure Exo‐rhamnogalacturonan Lyase Polysaccharide Lyase Family 26
Content Type	Text
Resource Type	Article

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