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Extraction of DNA from soil using nanoparticles by magnetic bioseparation
| Content Provider | Scilit |
|---|---|
| Author | Sebastianelli, A. Sen, Tapas Bruce, I. J. |
| Copyright Year | 2008 |
| Description | Journal: Letters in applied microbiology To develop a simple, rapid and inexpensive soil DNA extraction protocol.The protocol relies on the use of superparamagnetic silica-magnetite nanoparticles for the isolation and purification of DNA from soil samples. DNA suitable for use in molecular biology applications was obtained from a number of soil samples.The DNA extracted using the tested method successfully permitted the PCR amplification of a fragment of the bacterial 16S rDNA gene. The extracted DNA could also be restriction endonuclease digested.The protocol reported here is simple and permits rapid isolation of PCR-ready soil DNA. The method requires only small quantities of soil sample, is scalable and suitable for automation. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2008.02343.x/pdf |
| Ending Page | 491 |
| Page Count | 4 |
| Starting Page | 488 |
| e-ISSN | 1472765X |
| DOI | 10.1111/j.1472-765x.2008.02343.x |
| Journal | Letters in applied microbiology |
| Issue Number | 4 |
| Volume Number | 46 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2008-04-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Letters in applied microbiology Biochemical Research Magnetic Bioseparation Rapid Techniques |
| Content Type | Text |
| Resource Type | Article |
