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A Highly Selective Mitochondria-Targeting Fluorescent $K^{+}$Sensor
| Content Provider | Scilit |
|---|---|
| Author | Kong, Xiangxing Su, Fengyu Zhang, Liqiang Lee, Fred Shi, Zhengwei Tian, Yanqing Meldrum, Deirdre R. |
| Copyright Year | 2015 |
| Description | Journal: Angewandte Chemie International Edition Regulation of intracellular potassium (K(+) ) concentration plays a key role in metabolic processes. So far, only a few intracellular K(+) sensors have been developed. The highly selective fluorescent K(+) sensor KS6 for monitoring K(+) ion dynamics in mitochondria was produced by coupling triphenylphosphonium, borondipyrromethene (BODIPY), and triazacryptand (TAC). KS6 shows a good response to K(+) in the range 30-500 mM, a large dynamic range (Fmax /F0 ≈130), high brightness (ϕf =14.4 % at 150 mM of K(+) ), and insensitivity to both pH in the range 5.5-9.0 and other metal ions under physiological conditions. Colocalization tests of KS6 with MitoTracker Green confirmed its predominant localization in the mitochondria of HeLa and U87MG cells. K(+) efflux/influx in the mitochondria was observed upon stimulation with ionophores, nigericin, or ionomycin. KS6 is thus a highly selective semiquantitative K(+) sensor suitable for the study of mitochondrial potassium flux in live cells. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815426/pdf |
| Ending Page | 12057 |
| Page Count | 5 |
| Starting Page | 12053 |
| ISSN | 14337851 |
| e-ISSN | 15213773 |
| DOI | 10.1002/anie.201506038 |
| Journal | Angewandte Chemie International Edition |
| Issue Number | 41 |
| Volume Number | 54 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2015-08-21 |
| Access Restriction | Open |
| Subject Keyword | Journal: Angewandte Chemie International Edition Fluorescent Probes |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Catalysis |
