NDLI: Calcium-Induced Desensitization of Acetylcholine Release from Synaptosomes or Proteoliposomes Equipped with Mediatophore, a Presynaptic Membrane Protein

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Calcium-Induced Desensitization of Acetylcholine Release from Synaptosomes or Proteoliposomes Equipped with Mediatophore, a Presynaptic Membrane Protein

Content Provider	Scilit
Author	Israel, M. Meunier, F. M. Morel, N. Lesbats, B.
Copyright Year	1987
Description	Journal: Journal of Neurochemistry A "fatigue" of acetylcholine (ACh) release is described in cholinergic synaptosomes stimulated with the calcium ionophore A23187 or gramicidin. A small conditioning calcium entry, which did not trigger a large ACh release, led to a decrease of transmitter release elicited by a second large calcium influx. This fatigue was half-maximal at approximately 30 microM external calcium and developed in a few minutes. In contrast, activation of release by calcium was very rapid and was half-maximal at approximately 0.5 mM external calcium. Activation and desensitization of release could be attributed to the recently identified presynaptic membrane protein, the "mediatophore." Proteoliposomes equipped with purified mediatophore showed a calcium-dependent activation and "fatigue" of ACh release similar to that of synaptosomes. It was found that the ionophore A23187 rapidly equilibrated internal and external calcium concentrations in proteoliposomes. Thus, the external calcium concentration gave the internal concentration required for activation or desensitization of proteoliposomal ACh release. The mediatophore showed remarkable calcium binding properties (20 sites/molecule) with a KD of 25 microM. The physiological implications of desensitization on the organization of release sites are discussed.
Ending Page	982
Starting Page	975
ISSN	2573508X
e-ISSN	14714159
DOI	10.1111/j.1471-4159.1987.tb00989.x
Journal	Journal of Neurochemistry
Issue Number	3
Volume Number	49
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1987-09-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Neurochemistry Biochemical Research Biochemistry and Molecular Biology Acetylcholine Release Cholinergic Synaptosomes Presynaptic Membrane Protein
Content Type	Text
Resource Type	Article

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