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Structure and Synthesis of a Lipid-Containing Bacteriophage. Total Reconstitution of Bacteriophage PM2 in vitro
| Content Provider | Scilit |
|---|---|
| Author | Schäfer, Rolf Franklin, Richard M. |
| Copyright Year | 1978 |
| Description | Journal: Journal of Biological Inorganic Chemistry The lipid-containing bacteriophage PM2 was reconstituted stepwise from its purified denatured subunits. In the first step the nucleocapsid was reconstituted from the DNA and the two nucleocapsid proteins. Slight biochemical differences between reconstituted nucleocapsids and those isolated from native virus were seen. Combination of reconstituted nucleocapsid or nucleocapsid from virions with the coat and spike proteins in the presence of the viral lipids resulted in the formation of infectious virus in both cases. The reconstituted particles contained amounts of viral components similar to those in native virus, except for the lipid content. The amount of lipids present in the reconstituted particles was twice as high as the lipid content of native bacteriophage. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1978.tb12781.x/pdf |
| Ending Page | 596 |
| Page Count | 8 |
| Starting Page | 589 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1978.tb12781.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 2 |
| Volume Number | 92 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1978-12-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Bacteriophage Pm2 Total Reconstitution Lipid Containing Bacteriophage Pm2 in Vitro |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
