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Continuous capture of recombinant antibodies by ZnCl$ _{2}$ precipitation without polyethylene glycol
| Content Provider | Scilit |
|---|---|
| Author | Dutra, Gregory Komuczki, Daniel Jungbauer, Alois Satzer, Peter |
| Copyright Year | 2020 |
| Description | Journal: Engineering in Life Sciences The capture of recombinant antibodies from cell culture broth is the first critical step of downstream processing. We were able to develop a precipitation‐based method for the capture and purification of monoclonal antibodies based on divalent cations, namely $ZnCl_{2}$. Traditional precipitation processes have to deal with high dilution factors especially for resolubilization and higher viscosity due to the use of PEG as precipitation or co‐precipitation agent. By the use of the crosslinking nature of divalent cations without the use of PEG, we kept viscosity from the supernatant and resolubilization dilution factors very low. This is especially beneficial for the solid–liquid separation for the harvest and wash of the precipitate in continuous mode. For this harvest and wash, we used tangential flow filtration that benefits a lot from low viscosity solutions, which minimizes the membrane fouling. With this precipitation based on $ZnCl_{2,}$ we were able to implement a very lean and efficient process. We demonstrated precipitation studies with three different antibodies, Adalimumab, Trastuzumab, and Denosumab, and a continuous capture case study using tangential flow filtration for precipitate recovery. In this study, we achieved yields of 70%. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336134/pdf |
| Ending Page | 274 |
| Page Count | 10 |
| Starting Page | 265 |
| ISSN | 16180240 |
| e-ISSN | 16182863 |
| DOI | 10.1002/elsc.201900160 |
| Journal | Engineering in Life Sciences |
| Issue Number | 7 |
| Volume Number | 20 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2020-03-24 |
| Access Restriction | Open |
| Subject Keyword | Journal: Engineering in Life Sciences Biotechnology and Microbiology Downstream Processing |
| Content Type | Text |
| Resource Type | Article |
| Subject | Environmental Engineering Bioengineering Biotechnology |
