The bidirectional polar and unidirectional lateral flagellar motors of Vibrio alginolyticus are controlled by a single CheY species

Content Provider	Scilit
Author	Kojima, Masaru Kubo, Rumi Yakushi, Toshiharu Homma, Michio Kawagishi, Ikuro
Copyright Year	2007
Description	Journal: Molecular microbiology The bacterial flagellar motor is an elaborate molecular machine that converts ion-motive force into mechanical force (rotation). One of its remarkable features is its swift switching of the rotational direction or speed upon binding of the response regulator phospho-CheY, which causes the changes in swimming that achieve chemotaxis. Vibrio alginolyticus has dual flagellar systems: the Na(+)-driven polar flagellum (Pof) and the H(+)-driven lateral flagella (Laf), which are used for swimming in liquid and swarming over surfaces respectively. Here we show that both swimming and surface-swarming of V. alginolyticus involve chemotaxis and are regulated by a single CheY species. Some of the substitutions of CheY residues conserved in various bacteria have different effects on the Pof and Laf motors, implying that CheY interacts with the two motors differently. Furthermore, analyses of tethered cells revealed that their switching modes are different: the Laf motor rotates exclusively counterclockwise and is slowed down by CheY, whereas the Pof motor turns both counterclockwise and clockwise, and CheY controls its rotational direction.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2007.05623.x/pdf
Ending Page	67
Page Count	11
Starting Page	57
e-ISSN	13652958
DOI	10.1111/j.1365-2958.2007.05623.x
Journal	Molecular microbiology
Issue Number	1
Volume Number	64
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2007-03-16
Access Restriction	Open
Subject Keyword	Journal: Molecular microbiology Biochemistry and Molecular Biology
Content Type	Text
Resource Type	Article