NDLI: p-Chloromercuribenzoate Causes $Ca^{2+}$-Dependent Exocytotic Catecholamine Secretion from Cultured Bovine Adrenal Medullary Cells

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p-Chloromercuribenzoate Causes $Ca^{2+}$-Dependent Exocytotic Catecholamine Secretion from Cultured Bovine Adrenal Medullary Cells

Content Provider	Scilit
Author	Tachikawa, Eiichi Takahashi, Saburo Kashimoto, Takeshi
Copyright Year	1989
Description	Journal: Journal of Neurochemistry Incubation of cultured bovine adrenal medullary cells with p-chloromercuribenzoate (50-500 microM), a sulfhydryl-reacting agent, caused an increase in the secretion of catecholamines, p-Chloromercuriphenyl sulfonate, a p-chloromercuribenzoate analogue that poorly penetrates the cell membrane, caused a similar increase in catecholamine secretion. In both cases, catecholamine secretion was dependent on extracellular Ca2+. Furthermore, p-chloromercuribenzoate caused both 45Ca2+ influx into the cells and an increase in the intracellular free Ca2+ concentration. The increases in catecholamine secretion and 45Ca2+ influx behaved similarly in relation to p-chloromercuribenzoate concentration. The time courses of the increased secretion, 45Ca2+ influx, and intracellular free Ca2+ concentration by p-chloromercuribenzoate were also quite similar. The stimulation of catecholamine secretion by p-chloromercuribenzoate was reversed by washing the cells with dithiothreitol-containing medium, but not by dithiothreitol-free medium. When the cells were treated with p-chloromercuribenzoate, dopamine-beta-hydroxylase, an enzyme present in the chromaffin granules along with catecholamines, was also released. However, p-chloromercuribenzoate did not cause release of phenylethanolamine-N-methyltransferase, an enzyme present in the cytoplasm. These results indicate that catecholamine secretion due to p-chloromercuribenzoate occurs by Ca2+-dependent exocytosis.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1989.tb07290.x/pdf
Ending Page	26
Page Count	8
Starting Page	19
e-ISSN	14714159
DOI	10.1111/j.1471-4159.1989.tb07290.x
Journal	Journal of Neurochemistry
Issue Number	1
Volume Number	53
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1989-07-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Neurochemistry Bovine Adrenal Medullary Cells Catecholamine Secretion Intracellular Free Calcium Concentration P‐chloromercuribenzoate
Content Type	Text
Resource Type	Article

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