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Studies on Analogues of Succinic Semialdehyde as Substrates for Succinate Semialdehyde Dehydrogenase from Rat Brain
| Content Provider | Scilit |
|---|---|
| Author | Manzocchi, Ada Biondi, Pierantonio Secch, Camillo Santaniello, Enzo |
| Copyright Year | 2006 |
| Description | Journal: Journal of Neurochemistry The methyl ester of succinic semialdehyde (SSA) was examined as a substrate for succinate semialdehyde dehydrogenase (SSADH) from rat brain. It was found that the ester can be oxidized by the enzyme. Values of Km for SSA-Me were higher than for those for SSA, and for this substrate the enzyme showed a substrate-dependent inhibition. This finding suggests that the carboxylate group of SSA is not essential in the process of inhibition of SSADH by the substrate. Cyclopropyl analogues of SSA, cis- and trans-1-formyl-cyclopropan-2-carboxylic acids, were also individually tested as substrates of SSADH. Only the trans isomer was found to be oxidized to the corresponding dicarboxylic acid; it inhibited the enzyme in the same range of concentrations as SSA. The above data suggest that, as for gamma-aminobutyric acid, SSA is present in an unfolded, transoid conformation at the active site of SSADH. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1986.tb08510.x/pdf |
| Ending Page | 1898 |
| Page Count | 4 |
| Starting Page | 1895 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1986.tb08510.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 6 |
| Volume Number | 46 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2006-10-05 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Biochemistry and Molecular Biology Succinic Semialdehyde Succinic Semialdehyde Methyl Ester Succinate Semialdehyde Dehydrogenase 1‐formyl‐cyclopropan‐2‐carboxylic Acid |
| Content Type | Text |
| Resource Type | Article |
