NDLI: Meiosis induced by inactivation of Pat1 kinase proceeds with aberrant nuclear positioning of centromeres in the fission yeastSchizosaccharomyces pombe

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Meiosis induced by inactivation of Pat1 kinase proceeds with aberrant nuclear positioning of centromeres in the fission yeastSchizosaccharomyces pombe

Content Provider	Scilit
Author	Chikashige, Yuji Kurokawa, Rumi Haraguchi, Tokuko Hiraoka, Yasushi
Copyright Year	2004
Description	Journal: Genes to Cells Nuclear organization of chromosomes proceeds with significant changes during meiosis. In the fission yeast Schizosaccharomyces pombe, centromeres are clustered at the spindle-pole body (SPB) during the mitotic cell cycle; however, during meiotic prophase telomeres become clustered to the SPB and centromeres dissociate from the SPB. We followed the movement of telomeres, centromeres and sister chromatids in living S. pombe cells that were induced to meiosis by inactivation of Pat1 kinase (a key negative regulator of meiosis). Time-course observation in living cells determined the temporal order of DNA synthesis, telomere clustering, centromere separation and meiotic chromosome segregation. When meiosis was induced by Pat1 inactivation at the G1 phase of mitosis, telomeres clustered to the SPB as per normal meiosis, but in most cells the centromeres remained partially associated with the SPB. When meiosis was initiated at the G2 phase by Pat1 inactivation, both telomeres and centromeres retained their mitotic nuclear positions in the majority of cells. These results indicate that the progression of meiosis induced by Pat1 inactivation is aberrant from normal meiosis in some events. As Pat1 inactivation is often useful to induce S. pombe cells synchronously into meiosis, the temporal order of chromosomal events determined here will provide landmarks for the progression of meiosis downstream the Pat1 inactivation.
Ending Page	684
Starting Page	671
ISSN	2573508X
e-ISSN	13652443
DOI	10.1111/j.1356-9597.2004.00760.x
Journal	Genes to Cells
Issue Number	8
Volume Number	9
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2004-08-01
Access Restriction	Open
Subject Keyword	Journal: Genes to Cells Pat1 Inactivation Meiosis Induced Nuclear Positions
Content Type	Text
Resource Type	Article

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