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Developmentally regulated expression of matrix metalloproteinases during fetal rat colon morphogenesis
| Content Provider | Scilit |
|---|---|
| Author | Kurakata, Hidenori Oka, Masashi Matsubara, Yasuo Niwa, Tohru Utsunomiya, Hirotoshi Fujishiro, Mitsuhiro Miki, Kazumasa Fukamachi, Hiroshi Kubota, Syunichiro Ichinose, Masao |
| Copyright Year | 2007 |
| Description | Journal: Development, Growth & Differentiation To investigate the role of matrix metalloproteinases (MMPs) during gastrointestinal tract development, the expression of gelatinases (MMP-2 and MMP-9) was investigated during fetal rat colon morphogenesis. Fetal rat colons were separated into epithelial and mesenchymal fractions without cross contamination using a chelating agent and a dissecting microscope. Gelatinase activity measured using fluorescently labeled gelatin was higher in the mesenchymal than in the epithelial fraction; the developmental profile revealed that, in both fractions, gelatinase activity was enhanced during colon morphogenesis. During colonic gland formation, there was prominent MMP-2 activity, elevated MMP-2 mRNA expression, and an increase in the level of the active form of MMP-2 in the mesenchymal fraction. The mRNA expression of the tissue inhibitor of metalloproteinase 2 corresponded with an elevation in the level of the active form of MMP-2; the mRNA expression of the cell surface activator of MMP-2, membrane type matrix metalloproteinase 1, did not increase significantly. MMP-9 activity was low; only the pro-form was observed in the epithelial fraction at the end of fetal life. These results suggest that, during colon morphogenesis, MMP activity is under strict spatio-temporal control, and that the activity of MMP-2, which is regulated at both the transcriptional and proteolytic activation levels, is very much involved in rat colon morphogenesis. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1440-169X.2007.00971.x/pdf |
| Ending Page | 48 |
| Page Count | 8 |
| Starting Page | 41 |
| e-ISSN | 1440169X |
| DOI | 10.1111/j.1440-169x.2007.00971.x |
| Journal | Development, Growth & Differentiation |
| Issue Number | 1 |
| Volume Number | 50 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2007-11-06 |
| Access Restriction | Open |
| Subject Keyword | Journal: Development, Growth & Differentiation Gastroenterology and Hepatology Extracellular Matrix Matrix Metalloproteinase |
| Content Type | Text |
| Resource Type | Article |
