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Preconditioning-Mediated Neuroprotection
| Content Provider | Scilit |
|---|---|
| Author | Andoh, Tsugunobu Chock, P. Boon Chiueh, Chuang C. |
| Copyright Year | 2002 |
| Description | Journal: Annals of the New York Academy of Sciences Preconditioning adaptation induced by transient ischemia can increase brain tolerance to oxidative stress, but the underlying neuroprotective mechanisms are not fully understood. Recently, we developed a human brain-derived cell model to investigate preconditioning mechanism in SH-SY5Y neuroblastoma cells.(1) Our results demonstrate that a non-lethal serum deprivation-stress for 2 h (preconditioning stress) enhanced the tolerance to a subsequent lethal oxidative stress (24 h serum deprivation) and also to 1-methyl-4-phenyl-pyridinium (MPP(+)).(2) Two-hour non-lethal preconditioning stress increased the expression of neuronal nitric oxide (NOS1/nNOS) mRNA, Fos, Ref-1, NOS protein, and then nitric oxide (*NO) production. As well as MnSOD expression, the *NO-cGMP-PKG pathway mediated the preconditioning-induced upregulation of antiapoptotic protein Bcl-2 and the downregulation of adaptor protein p66(shc). We also propose that cGMP-mediated preconditioning-induced adaptation against oxidative stress may be due to the synthesis of a new protein, such as thioredoxin (Trx) since the protective effect can be blocked by Trx reductase inhibitor.(3) The antioxidative potency of Trx was approximately 100 and 1,000 times greater than GSNO and GSH, respectively. These results suggest that *NO-cGMP-PKG signaling pathway plays an important role in the preconditioning-induced neuroprotection, and perhaps cardioprotection, against oxidative stress. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1749-6632.2002.tb04051.x/pdf |
| Ending Page | 7 |
| Page Count | 7 |
| Starting Page | 1 |
| e-ISSN | 17496632 |
| DOI | 10.1111/j.1749-6632.2002.tb04051.x |
| Journal | Annals of the New York Academy of Sciences |
| Issue Number | 1 |
| Volume Number | 962 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2002-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Annals of the New York Academy of Sciences Preconditioning Mechanism |
| Content Type | Text |
| Resource Type | Article |
