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γ-Tubulin Ring Complexes and EB1 play antagonistic roles in microtubule dynamics and spindle positioning
| Content Provider | Scilit |
|---|---|
| Author | Bouissou, Anaїs Vérollet, Christel de Forges, Hélène Haren, Laurence Bellaїche, Yohanns Perez, Franck Merdes, Andreas Raynaud-Messina, Brigitte |
| Copyright Year | 2014 |
| Description | Journal: The EMBO Journal γ‐Tubulin is critical for microtubule (MT) assembly and organization. In metazoa, this protein acts in multiprotein complexes called γ‐Tubulin Ring Complexes (γ‐TuRCs). While the subunits that constitute γ‐Tubulin Small Complexes (γ‐TuSCs), the core of the MT nucleation machinery, are essential, mutation of γ‐TuRC‐specific proteins in Drosophila causes sterility and morphological abnormalities via hitherto unidentified mechanisms. Here, we demonstrate a role of γ‐TuRCs in controlling spindle orientation independent of MT nucleation activity, both in cultured cells and in vivo , and examine a potential function for γ‐TuRCs on astral MTs. γ‐TuRCs locate along the length of astral MTs, and depletion of γ‐TuRC‐specific proteins increases MT dynamics and causes the plus‐end tracking protein EB1 to redistribute along MTs. Moreover, suppression of MT dynamics through drug treatment or EB1 down‐regulation rescues spindle orientation defects induced by γ‐TuRC depletion. Therefore, we propose a role for γ‐TuRCs in regulating spindle positioning by controlling the stability of astral MTs. ![][1] γ‐TuRCs gain an unexpected mitotic function in stabilizing astral microtubules, independent of their essential microtubule nucleation roles at centrosomes EMBO Journal (2014) 33, 114–128 [1]: pending:yes |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989606/pdf |
| Ending Page | 128 |
| Page Count | 15 |
| Starting Page | 114 |
| ISSN | 02614189 |
| e-ISSN | 14602075 |
| DOI | 10.1002/embj.201385967 |
| Journal | The EMBO Journal |
| Issue Number | 2 |
| Volume Number | 33 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2014-01-14 |
| Access Restriction | Open |
| Subject Keyword | Journal: The EMBO Journal Gamma-tubulin Complexes Microtubule Dynamics Spindle Orientation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Immunology and Microbiology Medicine Molecular Biology |
