NDLI: Tripping the light fantastic in membrane redox biology: linking dynamic structures to function in ER electron transfer chains

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Tripping the light fantastic in membrane redox biology: linking dynamic structures to function in ER electron transfer chains

Content Provider	Scilit
Author	Hedison, Tobias Scrutton, Nigel S.
Copyright Year	2019
Description	Journal: The FEBS Journal How the dynamics of proteins assist catalysis is a contemporary issue in enzymology. In particular, this holds true for membrane‐bound enzymes, where multiple structural, spectroscopic and biochemical approaches are needed to build up a comprehensive picture of how dynamics influence enzyme reaction cycles. Of note are the recent studies of cytochrome P450 reductases (CPR)–P450 (CYP) endoplasmic reticulum redox chains, showing the relationship between dynamics and electron flow through flavin and haem redox centres and the impact this has on monooxygenation chemistry. These studies have led to deeper understanding of mechanisms of electron flow, including the timing and control of electron delivery to protein‐bound cofactors needed to facilitate CYP‐catalysed reactions. Individual and multiple component systems have been used to capture biochemical behaviour and these have led to the emergence of more integrated models of catalysis. Crucially, the effects of membrane environment and composition on reaction cycle chemistry have also been probed, including effects on coenzyme binding/release, thermodynamic control of electron transfer, conformational coupling between partner proteins and vectorial versus ‘off pathway’ electron flow. Here, we review these studies and discuss evidence for the emergence of dynamic structural models of electron flow along human microsomal CPR–P450 redox chains.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563164/pdf
Ending Page	2017
Page Count	14
Starting Page	2004
e-ISSN	17424658
DOI	10.1111/febs.14757
Journal	The FEBS Journal
Issue Number	11
Volume Number	286
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2019-01-30
Access Restriction	Open
Subject Keyword	Journal: The FEBS Journal Biochemistry and Molecular Biology Cytochrome P450 Cytochrome P450 Reductase Electron Transfer Chemistry Membrane Protein Protein Domain Dynamics
Content Type	Text
Resource Type	Article

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