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Purification and characterization of cystathionine gamma-synthase type II from Bacillus sphaericus
| Content Provider | Scilit |
|---|---|
| Author | Kanzaki, Hiroshi Kobayashi, Michihiko Nagasawa, Toru Yamada, Hideaki |
| Copyright Year | 1987 |
| Description | Journal: Journal of Biological Inorganic Chemistry Cystathionine gamma-synthase type II, which catalyzes L-cystathionine synthesis from O-acetyl-L-homoserine and L-cysteine was purified from Bacillus sphaericus (IFO 3536) in seven steps. The purified enzyme appeared to be homogeneous by the results of polyacrylamide electrophoresis and ampholyte electrofocusing. The enzyme is a typical pyridoxal-P dependent enzyme, has a molecular mass of 165 kDa and consists of four subunits identical in molecular mass. The enzyme catalyzed the gamma-replacement reaction and the elimination reaction was hardly detected even when a large amount of enzyme was added. In the replacement reaction, O-acetyl-L-homoserine and the following thiol compounds: L and D-cysteine, L and D-homocysteine, sodium sulfide, various alkyl and aryl mercaptans, acted as the most suitable substrate to produce L-cystathionine and the corresponding S-substituted L-homocysteine derivatives. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1987.tb10742.x/pdf |
| Ending Page | 112 |
| Page Count | 8 |
| Starting Page | 105 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1987.tb10742.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 163 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1987-02-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biotechnology and Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
