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A rapid, inexpensive and disposable point-of-care blood test for sickle cell disease using novel, highly specific monoclonal antibodies
| Content Provider | Scilit |
|---|---|
| Author | Quinn, Charles T. Paniagua, Mary C. DiNello, Robert K. Panchal, Anand Geisberg, Mark |
| Copyright Year | 2016 |
| Description | Journal: British Journal of Haematology Sickle cell disease (SCD) is a significant healthcare burden worldwide, but most affected individuals reside in low-resource areas where access to diagnostic testing may be limited. We developed and validated a rapid, inexpensive, disposable diagnostic test, the HemoTypeSC™ , based on novel monoclonal antibodies (MAbs) that differentiate normal adult haemoglobin (Hb A), sickle haemoglobin (Hb S) and haemoglobin C (Hb C). In competitive enzyme-linked immunosorbent assays, each MAb bound only its target with <0·1% cross-reactivity. With the HemoTypeSC™ test procedure, the sensitivity for each variant was <5·0 g/l. The accuracy of HemoTypeSC™ was evaluated on 100 whole blood samples from individuals with common relevant haemoglobin phenotypes, including normal (Hb AA, N = 20), carrier or trait (Hb AS, N = 22; Hb AC, N = 20), SCD (Hb SS, N = 22; Hb SC, N = 13), and Hb C disease (Hb CC, N = 3). The correct haemoglobin phenotype was identified in 100% of these samples. The accuracy of the test was not affected by Hb F (0-94·8% of total Hb) or Hb A2 (0-5·6% of total Hb). HemoTypeSC™ requires <1 μl of whole blood and no instruments or power sources. The total time-to-result is <20 min. HemoTypeSC™ may be a practical solution for point-of-care testing for SCD and carrier status in low-resource settings. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118141/pdf |
| Ending Page | 732 |
| Page Count | 9 |
| Starting Page | 724 |
| e-ISSN | 13652141 |
| DOI | 10.1111/bjh.14298 |
| Journal | British Journal of Haematology |
| Issue Number | 4 |
| Volume Number | 175 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2016-09-08 |
| Access Restriction | Open |
| Subject Keyword | Journal: British Journal of Haematology Haemoglobinopathy Lateral Flow Immunoassay Monoclonal Antibody Sickle Cell Disease |
| Content Type | Text |
| Resource Type | Article |
