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Cooperative Modulation of $[^{3}$H]MK-801 Binding to the N-Methyl-d-Aspartate Receptor-Ion Channel Complex by l-Glutamate, Glycine, and Polyamines
| Content Provider | Scilit |
|---|---|
| Author | Ransom, Richard W. Stec, Nancy L. |
| Copyright Year | 1988 |
| Description | Journal: Journal of Neurochemistry In extensively washed rat cortical membranes $[^{3}$ H](+)- 5 - methyl - 10,11 - dihydro - 5 H- dibenzo [a,d]cyclohepten-5,10-imine $([^{3}$H]MK-801) labeled a homogeneous set of sites $(B_{max}$=1.86 pmol/mg protein) with relatively low affinity $(K_{D}$= 45 nM). l-Glutamate, glycine. and spermidine produced concentration-dependent increases in specific $[^{3}$H]MK-801 binding due to a reduction in the $K_{d}$ of the radioligand. In the presence of high concentrations of l-glutamate, glycine, or spermidine, the $K_{D}$ values for $[^{3}$H]MK-801 were reduced to 11 nM, 18 nM, and 15 nM, respectively. Maximally effective concentrations of combinations of the three compounds further increased $[^{3}$H]MK-801 binding affinity as follows: l-glutamate + glycine, $K_{D}$= 6.2 nM; l-glutamate + spermidine, $K_{D}$= 2.2 nM: |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.1988.tb01818.x |
| Ending Page | 836 |
| Page Count | 7 |
| Starting Page | 830 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1988.tb01818.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 3 |
| Volume Number | 51 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1988-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Biochemistry and Molecular Biology N-methyl-d-aspartate Receptor |
| Content Type | Text |
| Resource Type | Article |
