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Glycine-Like Modulation of N-Methyl-d-Aspartate Receptors by a Monoclonal Antibody That Enhances Long-Term Potentiation
| Content Provider | Scilit |
|---|---|
| Author | Haring, Rachel Stanton, Patric K. Scheideler, Mark A. Moskal, Joseph R. |
| Copyright Year | 1991 |
| Description | Journal: Journal of Neurochemistry We have identified a monoclonal antibody, B6B21, that significantly elevates long-term potentiation when applied to CA1 pyramidal cell apical dendrites in rat hippocampal slices and characterized its binding to N-methyl-D-aspartate-receptor complexes using extensively washed hippocampal membranes. Five micrograms of affinity-purified B6B21 per 100 micrograms of membranes gave a two- to threefold elevation in N-[1-(2-thienyl)cyclohexyl]-3,4-[3H]piperidine ([3H]TCP) binding. When [3H]TCP binding was stimulated by the combined addition of maximal concentrations of glutamate, glycine, and magnesium, B6B21 no longer stimulated [3H]TCP binding. Like glycine, B6B21 enhanced the effect of N-methyl-D-aspartate and glutamate in stimulating [3H]TCP binding. Moreover, B6B21 reversed 7-chlorokynurenic acid inhibition of [3H]TCP binding, but it had no effect on the inhibition of [3H]TCP binding by D-(-)-2-amino-5-phosphonovaleric acid. B6B21 increased the rate of association and dissociation of [3H]TCP, but had no effect on equilibrium binding. Glutamate, but not glycine, however, increased B6B21-enhancement of [3H]TCP association and dissociation. B6B21 binding at strychnine-insensitive glycine sites was confirmed by direct measurement of [3H]glycine binding. These results suggest that B6B21 binds directly to N-methyl-D-aspartate receptors and displays properties similar to glycine. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1991.tb02131.x/pdf |
| Ending Page | 332 |
| Page Count | 10 |
| Starting Page | 323 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1991.tb02131.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 1 |
| Volume Number | 57 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1991-07-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Biochemistry and Molecular Biology N‐methyl‐d‐aspartate Receptors‐long‐term Potentiation Phencyclidine Receptor N‐[1‐(2‐thienyl)cyclohexyl‐3,4‐[3h]piperidine Binding Glycine Recognition Sites [3h]glycine Binding |
| Content Type | Text |
| Resource Type | Article |
