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Purification and Characterization of a Ribonuclease from Aspergillus giganteus IFO 5818, the Gigantin. Immunological and Enzymic Comparison with Alphasarcin
| Content Provider | Scilit |
|---|---|
| Author | Salvarelli, Stephanie Munoz, Saturnino Conde, Francisco Pedro |
| Copyright Year | 1994 |
| Description | Journal: Journal of Biological Inorganic Chemistry A protein has been isolated from a culture medium of Aspergillus giganteus IFO 5818 (gigantin) and purified by ion-exchange chromatography successively on DEAE-cellulose and carboxymethyl-cellulose, and gel-filtration chromatography on Biogel P10. With a high purity, gigantin was found to be a non-glycosylated basic protein with a relative molecular mass of 17000±200 determined in PAGE/SDS. Gigantin was able to digest the synthetic homopolymers of nucleic acids poly(A), poly(I), poly(C) and poly(U). The catalytic action has an optimal pH around 7.0, an optimal temperature at 45–55°C and can be inhibited by cations. Gigantin activity, analyzed as its capacity to hydrolyze RNA from yeast, was comparable to that of alphasarcin, a similar biochemical protein produced by the strain A. giganteus MDH 18894. A study of the substrate specificity for alphasarcin indicated a preference for poly(A) and poly(I), while gigantin had greater activity on poly(C) and poly(U). The cross reaction of gigantin with a rabbit antiserum to alphasarcin suggests a high sequence similarity between both proteins. However, gigantin is immunologically distinguishable from alphasarcin as alphasarcin antiserum detects epitopes in alphasarcin that are not present in gigantin. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1994.00243.x/pdf |
| Ending Page | 251 |
| Page Count | 9 |
| Starting Page | 243 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1994.00243.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 225 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1994-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
