Loading...
Please wait, while we are loading the content...
Similar Documents
Covalent binding of an NAD+ analogue to horse liver alcohol dehydrogenase in a ternary complex with pyrazole
| Content Provider | Scilit |
|---|---|
| Author | Goulas, Philippe |
| Copyright Year | 1987 |
| Description | Journal: Journal of Biological Inorganic Chemistry Examination of the model of the fixation site of the adenosine phosphate part of NAD+ on horse liver alcohol dehydrogenase led us to synthesize a NAD+ analogue N6-[N-(8-amino-3,6-dioxaoctyl)carbamoylmethyl]-NAD+ in order to alkylate the carboxylic acid group of Asp-273 and to convert the normally dissociable coenzyme into a permanently bound prosthetic group. This NAD+ analogue is coupled to the horse liver alcohol dehydrogenase in the ternary complex formed with pyrazole. In these conditions the degree of fixation varies between 0.4 and 0.58 coenzyme molecule/enzyme subunit molecule. The N6-[N-(8-amino-3,6-dioxaoctyl)carbamoylmethyl]NAD+ acts as a true prosthetic group which can be reduced and reoxidized by a coupled substrate reaction and the internal activity of this holoenzyme corresponds to the amount of analogue incorporated. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1987.tb13440.x/pdf |
| Ending Page | 473 |
| Page Count | 5 |
| Starting Page | 469 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1987.tb13440.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 2 |
| Volume Number | 168 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1987-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Medicinal Chemistry Horse Liver Alcohol Liver Alcohol Dehydrogenase |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
