NDLI: Engineering Three‐Dimensional Collagen Matrices to Provide Contact Guidance during 3D Cell Migration

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Engineering Three‐Dimensional Collagen Matrices to Provide Contact Guidance during 3D Cell Migration

Content Provider	Scilit
Author	Provenzano, Paolo P. Eliceiri, Kevin W. Inman, David R. Keely, Patricia J.
Copyright Year	2010
Description	Journal: Current Protocols in Cell Biology Cell invasion requires that cells navigate complex three‐dimensional matrices in vivo. Topological cues are provided and three‐dimensional cell migration and invasion facilitated by the alignment of collagen fibers proximal to tumors. In order to better understand the molecular mechanisms by which cells recognize and migrate along aligned matrices, in vitro assays are needed that recapitulate topological features of the in vivo matrix. Here, we describe two approaches for creating aligned three‐dimensional collagen matrices, both dependent and independent of cell‐mediated alignment. Approaches to quantify alignment and visualize the collagen matrix relative to the cells by second‐harmonic generation are included. These assays are readily adaptable to a variety of cells and biological questions related to three‐dimensional cell migration. Curr. Protoc. Cell Biol. 47:10.17.1‐10.17.11. © 2010 by John Wiley & Sons, Inc.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905986/pdf
ISSN	19342500
e-ISSN	19342616
DOI	10.1002/0471143030.cb1017s47
Journal	Current Protocols in Cell Biology
Issue Number	1
Volume Number	47
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2010-06-01
Access Restriction	Open
Subject Keyword	Journal: Current Protocols in Cell Biology Developmental Biology Collagen Alignment Three‐dimensional Cell Migration
Content Type	Text
Subject	Cell Biology Medicine

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