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Substrate specificity of a recombinant d-lyxose isomerase from Serratia proteamaculans that produces d-lyxose and d-mannose
| Content Provider | Scilit |
|---|---|
| Author | Park, C. -S. Yeom, S. -J. Lim, Y. -R. Kim, Y. -S. Oh, D. -K. |
| Copyright Year | 2010 |
| Description | Journal: Letters in applied microbiology Characterization of substrate specificity of a D-lyxose isomerase from Serratia proteamaculans and application of the enzyme in the production of D-lyxose and D-mannose. The concentrations of monosaccharides were determined using a Bio-LC system. The activity of the recombinant protein from Ser. proteamaculans was the highest for D-lyxose among aldoses, indicating that it is a D-lyxose isomerase. The native recombinant enzyme existed as a 54-kDa dimer, and the maximal activity for D-lyxose isomerization was observed at pH 7.5 and 40 degrees C in the presence of 1 mmol l(-1) Mn(2+). The K(m) values for D-lyxose, D-mannose, D-xylulose, and D-fructose were 13.3, 32.2, 3.83, and 19.4 mmol l(-1), respectively. In 2 ml of reaction volume at pH 7.5 and 35 degrees C, D-lyxose was produced at 35% (w/v) from 50% (w/v) D-xylulose by the D-lyxose isomerase in 3 h, while D-mannose were produced at 10% (w/v) from 50% (w/v) D-fructose in 5 h. We identified the putative sugar isomerase from Ser. proteamaculans as a D-lyxose isomerase. The enzyme exhibited isomerization activity for aldose substrates with the C2 and C3 hydroxyl groups in the left-hand configuration. High production rates of d-lyxose and d-mannose by the enzyme were obtained. A new D-lyxose isomerase was found, and this enzyme had higher activity for D-lyxose and D-mannose than previously reported enzymes. Thus, the enzyme can be applied in industrial production of D-lyxose and D-mannose. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02903.x/pdf |
| Ending Page | 350 |
| Page Count | 8 |
| Starting Page | 343 |
| e-ISSN | 1472765X |
| DOI | 10.1111/j.1472-765x.2010.02903.x |
| Journal | Letters in applied microbiology |
| Issue Number | 3 |
| Volume Number | 51 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2010-08-16 |
| Access Restriction | Open |
| Subject Keyword | Journal: Letters in applied microbiology Biotechnology and Microbiology D‐lyxose Isomerase D‐lyxose Production D‐mannose Production Serratia Proteamaculans Substrate Specificity |
| Content Type | Text |
| Resource Type | Article |
