NDLI: Terminal Alkenes from Acrylic Acid Derivatives via Non-Oxidative Enzymatic Decarboxylation by Ferulic Acid Decarboxylases

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Terminal Alkenes from Acrylic Acid Derivatives via Non-Oxidative Enzymatic Decarboxylation by Ferulic Acid Decarboxylases

Content Provider	Scilit
Author	Aleku, Godwin A. Prause, Christoph Allen, Ruth Bradshaw Plasch, Katharina Glueck, Silvia M. Bailey, Samuel S. Payne, Karl A. P. Parker, David A. Faber, Kurt Leys, David
Copyright Year	2018
Description	Journal: ChemCatChem Fungal ferulic acid decarboxylases (FDCs) belong to the UbiD‐family of enzymes and catalyse the reversible (de)carboxylation of cinnamic acid derivatives through the use of a prenylated flavin cofactor. The latter is synthesised by the flavin prenyltransferase UbiX. Herein, we demonstrate the applicability of FDC/UbiX expressing cells for both isolated enzyme and whole‐cell biocatalysis. FDCs exhibit high activity with total turnover numbers (TTN) of up to 55000 and turnover frequency (TOF) of up to 370 min‐1. Co‐solvent compatibility studies revealed FDC's tolerance to some organic solvents up 20% v/v. Using the in‐vitro (de)carboxylase activity of holo‐FDC as well as whole‐cell biocatalysts, we performed a substrate profiling study of three FDCs, providing insights into structural determinants of activity. FDCs display broad substrate tolerance towards a wide range of acrylic acid derivatives bearing (hetero)cyclic or olefinic substituents at C3 affording conversions of up to >99%. The synthetic utility of FDCs was demonstrated by a preparative‐scale decarboxylation
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175315/pdf
Ending Page	3745
Page Count	10
Starting Page	3736
ISSN	18673880
e-ISSN	18673899
DOI	10.1002/cctc.201800643
Journal	ChemCatChem
Issue Number	17
Volume Number	10
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2018-09-07
Access Restriction	Open
Subject Keyword	Journal: ChemCatChem Biotechnology and Microbiology Ferulic Acid Decarboxylase Prenylated Flavin
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Physical and Theoretical Chemistry Catalysis Inorganic Chemistry

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