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NOE and two-dimensional correlated 1H-NMR spectroscopy of cytochrome c' from Chromatium vinosum
| Content Provider | Scilit |
|---|---|
| Author | Banci, Lucia Bertini, Ivano Turano, Paola Oliver, Margarita Vicens |
| Copyright Year | 1992 |
| Description | Journal: Journal of Biological Inorganic Chemistry 1H two-dimensional (nuclear Overhauser effect spectroscopy (NOESY) and two-dimensional correlated spectroscopy (COSY) spectra of cytochrome c' from Chromatium vinosum have been obtained. The protein is of medium size (Mr 28,000), essentially high spin (S = 5/2) although some quantum mechanical spin admixing with S = 3 2 may be present. Under these circumstances NOESY cross peaks have been revealed between geminal protons (alpha-CH2 propionate and beta-CH2 protons of the bound histidine) and between alpha-CH2 propionate protons and the heme methyl groups. COSY maps have confirmed the geminal nature of the proton pairs, even with a linewidth as large as 900 Hz; the J value is about 12 Hz. This assignment has rationalized on a sound basis the biochemical behavior of this protein with pH and has showed the utility of this kind of spectroscopy for the other cytochromes c' structures and analogous systems. |
| Ending Page | 112 |
| Starting Page | 107 |
| ISSN | 2573508X |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1992.tb16611.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 204 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1992-02-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Nmr Spectroscopy 1h Nmr Chromatium Vinosum Correlated 1h |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
