NDLI: Monoclonal antibodies directed against the human A431 beta-adrenergic receptor recognize two major polypeptide chains

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Monoclonal antibodies directed against the human A431 beta-adrenergic receptor recognize two major polypeptide chains

Content Provider	Scilit
Author	Kaveri, Srinivas V. Cervantes-Olivier, Paulina Delavier-Klutchko, Colette Strosberg, A. Donny
Copyright Year	1987
Description	Journal: Journal of Biological Inorganic Chemistry A serum-albumin-alprenolol conjugate was used to isolate beta-adrenergic receptors from the human A431 cell lysates. Three monoclonal antibodies were obtained from BALB/c mice immunized with these receptors. These antibodies: BRK-1, BRK-2, BRK-3, were respectively of the IgM, IgG2a and IgG3 classes. All three antibodies recognized photoaffinity-labelled receptors, immunoprecipitated ligand-binding activity and identified the 65-kDa and 55-kDa polypeptides corresponding to the beta 2-adrenergic receptors of A431 cells. BRK-2 and BRK-3 recognized both beta 1 and beta 2-adrenergic receptors of several mammalian cells. All three antibodies visualized, by immunofluorescence, the beta 2-adrenergic receptors at the surface of A431 cells. The monoclonal antibodies are directed against the protein portion of the beta-adrenergic receptors since partial or complete removal of the carbohydrate moieties by treatment with endoglycosidase such as endo-F (endo-beta-N-acetylglucosaminidase F) and periodate oxidation did not affect the immunoreactivity. These antibodies will be of value to immunopurify the beta-adrenergic receptors.
Ending Page	456
Starting Page	449
ISSN	2573508X
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1987.tb13358.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	3
Volume Number	167
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1987-09-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Analytical Chemistry Monoclonal Antibody Adrenergic Receptor
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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