Loading...
Please wait, while we are loading the content...
Similar Documents
Misregulation of poly(ADP-ribose) polymerase-1 activity and cell type-specific loss of poly(ADP-ribose) synthesis in the cerebellum of aged rats
| Content Provider | Scilit |
|---|---|
| Author | Malanga, M. Romano, M. Ferone, A. Petrella, A. Monti, G. Jones, R. Limatola, E. Farina, B. Monti, Maria Gaia |
| Copyright Year | 2005 |
| Description | Journal: Journal of Neurochemistry Protein modification by ADP-ribose polymers is a common regulatory mechanism in eukaryotic cells and is involved in several aspects of brain physiology and physiopathology, including neurotransmission, memory formation, neurotoxicity, ageing and age-associated diseases. Here we show age-related misregulation of poly(ADP-ribose) synthesis in rat cerebellum as revealed by: (i) reduced poly(ADP-ribose) polymerase-1 (PARP-1) activation in response to enzymatic DNA cleavage, (ii) altered protein poly(ADP-ribosyl)ation profiles in isolated nuclei, and (iii) cell type-specific loss of poly(ADP-ribosyl)ation capacity in granule cell layer and Purkinje cells in vivo. In particular, although PARP-1 could be detected in virtually all granule cells, only a fraction of them appeared to be actively engaged in poly(ADP-ribose) synthesis and this fraction was reduced in old rat cerebellum. NAD(+), quantified in tissue homogenates, was essentially the same in the cerebellum of young and old rats suggesting that in vivo factors other than PARP-1 content and/or NAD(+) levels may be responsible for the age-associated lowering of poly(ADP-ribose) synthesis. Moreover, PARP-1 expression was substantially down-regulated in Purkinje cells of senescent rats. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2005.03082.x/pdf |
| Ending Page | 1009 |
| Page Count | 10 |
| Starting Page | 1000 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.2005.03082.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 4 |
| Volume Number | 93 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2005-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Poly(adp‐ribose)polymerase‐1 Poly(adp‐ribosyl)ation |
| Content Type | Text |
| Resource Type | Article |
