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Inhibition of Lymphocyte Proliferation by Ovine Trophoblast Protein-1 and a High Molecular Weight Glycoprotein Produced by the Peri-Implantation Sheep Conceptus
| Content Provider | Scilit |
|---|---|
| Author | Newton, G. R. Vallet, J. L. Hansen, P. J. Bazer, F. W. |
| Copyright Year | 1989 |
| Description | Journal: American Journal of Reproductive Immunology Sheep conceptuses were flushed from uteri on day 16 of pregnancy and cultured in vitro. Three peaks of immunosuppressive activity (i.e., ability to inhibit $[^{3}$H]thymidine incorporation into mitogen-stimulated lymphocytes) were localized in conceptus-conditioned culture medium: one corresponding to a high-molecular-weight glycoprotein (HMWG; Mr = 800–900 kDa), one corresponding to ovine trophoblast protein-1 (oTP-1), the antiluteolytic, interferon-like molecule of the sheep conceptus, and a third fraction containing a previously undescribed molecule with a molecular weight between 10 and 14 kDa. Both HMWG and oTP-1 inhibited lymphocyte proliferation in a dose-dependent manner. Delaying the addition of HMWG or oTP-1 until 24 h after the addition of phytohemagglutinin (PHA) reduced the degree of suppression relative to cultures where HMWG and oTP-1 were added at the time of PHA addition. Nonetheless, the molecules were still capable of inhibiting proliferation. Addition of human recombinant IL-2 (0–65 U/ml) to PHA-stimulated lymphocyte cultures could not reverse HMWG- or oTP-1-induced suppression of $[^{3}$H]thymidine uptake by PHA-stimulated cells. Furthermore, treatment of lymphocytes with HMWG or oTP-1 suppressed IL-2-induced proliferation. Therefore, HMWG and oTP-1 affect both early and later events in the in vitro proliferative response of mitogen-stimulated lymphocytes, including responsiveness to IL-2. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0897.1989.tb00556.x |
| e-ISSN | 16000897 |
| DOI | 10.1111/j.1600-0897.1989.tb00556.x |
| Journal | American Journal of Reproductive Immunology |
| Issue Number | 3 |
| Volume Number | 19 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1989-03-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: American Journal of Reproductive Immunology Reproductive Biology Immunosuppression |
| Content Type | Text |
| Resource Type | Article |
