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Co-expression of TNFR2 and CD25 identifies more of the functional $CD4^{+}FOXP3^{+}$regulatory T cells in human peripheral blood
| Content Provider | Scilit |
|---|---|
| Author | Chen, Xin Subleski, Jeffrey J. Hamano, Ryoko Howard, O. M. Zack Wiltrout, Robert H. Oppenheim, Joost J. |
| Copyright Year | 2010 |
| Description | Journal: European Journal of Immunology Previously, we found that co‐expression of CD25 and TNFR2 identified the most suppressive subset of mouse Treg. In this study, we report that human peripheral blood (PB) $FOXP3^{+}$ cells present in $CD25^{high}$, $CD25^{low}$ and even $CD25^{–}$ subsets of $CD4^{+}$ cells expressed high levels of TNFR2. Consequently, TNFR2‐expressing $CD4^{+}CD25^{+}$ Treg included all of the $FOXP3^{+}$ cells present in the $CD4^{+}CD25^{high}$ subset as well as a substantial proportion of the $FOXP3^{+}$ cells present in the $CD4^{+}CD25^{low}$ subset. Flow cytometric analysis of PB identified five‐fold more Treg, determined by FOXP3 expression, in the $CD4^{+}CD25^{+}TNFR2^{+}$ subset than in the $CD4^{+}CD25^{high}$ subset. In addition, similar levels of $FOXP3^{+}$ cells were identified in both the $CD4^{+}CD25^{+}TNFR2^{+}$ and $CD4^{+}CD25^{+}CD127^{low/−}$ subsets. Furthermore, the $CD4^{+}CD25^{+}TNFR2^{+}$ subset expressed high levels of CTLA‐4, CD45RO, CCR4 and low levels of CD45RA and CD127, a phenotype characteristic of Treg. Upon TCR stimulation, human PB $CD4^{+}CD25^{+}TNFR2^{+}$ cells were anergic and markedly inhibited the proliferation and cytokine production of co‐cultured T‐responder cells. In contrast, $CD4^{+}CD25^{+}TNFR2^{–}$ and $CD4^{+}CD25^{–}TNFR2^{+}$ T cells did not show inhibitory activity. As some non‐Treg express TNFR2, the combination of CD25 and TNFR2 must be used to identify a larger population of human Treg, a population that may prove to be of diagnostic and therapeutic benefit in cancer and autoimmune diseases. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096013/pdf |
| Ending Page | 1106 |
| Page Count | 8 |
| Starting Page | 1099 |
| e-ISSN | 15214141 |
| DOI | 10.1002/eji.200940022 |
| Journal | European Journal of Immunology |
| Issue Number | 4 |
| Volume Number | 40 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2010-02-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: European Journal of Immunology Human Regulatory T Cells Peripheral Blood |
| Content Type | Text |
| Resource Type | Article |
