NDLI: Anterograde-propagation of axonal degeneration in the visual system of wlds mice characterized by diffusion tensor imaging

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Anterograde-propagation of axonal degeneration in the visual system of wlds mice characterized by diffusion tensor imaging

Content Provider	Scilit
Author	Sun, Shu-Wei Nishioka, Christopher Chung, Chen-Fang Park, Joann Liang, Hsiao-Fang
Copyright Year	2016
Description	Journal: Journal of Magnetic Resonance Imaging (JMRI) To evaluate the feasibility of using Diffusion Tensor Imaging (DTI) to characterize the temporospatial profile of axonal degeneration and its relation to blood-brain-barrier (BBB) permeability. Longitudinal DTI was performed in Wallerian degeneration slow (WldS) mice following retinal ischemia. In parallel, Gadolinium (Gd)-enhanced T1-weighted imaging (Gd-T1WI) was performed to evaluate BBB permeability in white matter during axonal degeneration. To confirm the in vivo findings, immunohistochemistry using SMI-31 and MBP was performed to examine the axons and myelin, respectively, and Evans Blue was used to evaluate the permeability of the BBB. Reduced axial diffusivity was found in the optic nerve (ON, −15%, p=0.0063) one week and optic tact (OT, −18%, p=0.0077) two weeks after retinal ischemia, which were respectively associated with an 11% (p=0.0116) and 25% (p=0.0001) axonal loss. Increased radial diffusivity was found 1–2 weeks after the co-located decrease of axial diffusivity (35% increase, p = 0.0388 in the ON at week 2 and an 80% increase, p = 0.0015 in the OT at week 4). No significant changes were observed using Gd-T1WI (p = 0.13 −0.75), although an approximately 1-fold increase in Evans blue staining intensity was found in the injured ON and OT starting one week after retinal ischemia. We demonstrated the utility of DTI to characterize anterograde-propagating axonal degeneration through the ON and OT following retinal damage. Evans blue staining revealed serum albumin accumulation at injured sites, though there was no BBB leakage detectable using Gd-T1WI.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426298/pdf
Ending Page	491
Page Count	10
Starting Page	482
e-ISSN	15222586
DOI	10.1002/jmri.25368
Journal	Journal of Magnetic Resonance Imaging (JMRI)
Issue Number	2
Volume Number	45
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2016-07-04
Access Restriction	Open
Subject Keyword	Journal: Journal of Magnetic Resonance Imaging (JMRI) Radiology, Nuclear Medicine and Imaging Diffusion Tensor Imaging (dti) Wallerian Degeneration
Content Type	Text
Resource Type	Article

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