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Development of detection medium for hard-to-culture beer-spoilage lactic acid bacteria
| Content Provider | Scilit |
|---|---|
| Author | Suzuki, K. Asano, S. Iijima, K. Kuriyama, H. Kitagawa, Y. |
| Copyright Year | 2008 |
| Description | Journal: Journal of Applied Microbiology To develop a detection medium for hard-to-culture beer-spoilage lactic acid bacteria (LAB). Four hard-to-culture beer-spoilage strains of LAB, belonging to Lactobacillus paracollinoides and Lactobacillus lindneri, have been obtained by repeatedly subculturing the wild-type strains in beer. To develop a countermeasure against these hard-to-culture beer-spoilage LAB, a beer-based medium was modified. As a consequence, the supplementation of a small amount of de Man Rogosa Sharpe medium was found to enhance the growth of hard-to-culture beer-spoilage LAB strains obtained in this study. In addition, sodium acetate was shown to improve the selectivity of this beer-based medium. Further comparative study was performed with five other media widely used for the detection of beer-spoilage LAB in the brewing industry. This study revealed that the newly developed medium, designated advanced beer-spoiler detection (ABD) medium, possessed superior sensitivity for hard-to-culture beer-spoilage LAB and comparable sensitivity with easy-to-culture beer-spoilage LAB. Moreover, ABD medium was found to suppress the growth of nonspoilage micro-organisms, and thereby allow the selective growth of beer-spoilage LAB. Advanced beer-spoiler detection medium is considered as an effective tool for comprehensive detection of beer-spoilage LAB in breweries. The detection by ABD medium can be used as an indicator for differentiating the beer-spoilage ability of LAB without further confirmatory tests in breweries. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2007.03669.x/pdf |
| Ending Page | 1470 |
| Page Count | 13 |
| Starting Page | 1458 |
| e-ISSN | 13652672 |
| DOI | 10.1111/j.1365-2672.2007.03669.x |
| Journal | Journal of Applied Microbiology |
| Issue Number | 5 |
| Volume Number | 104 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2008-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Applied Microbiology Analytical Chemistry Advanced Beer‐spoiler Detection Medium Beer Spoilage Detection Medium Lactic Acid Bacteria Lactobacillus Lindneri Lactobacillus Paracollinoides |
| Content Type | Text |
| Resource Type | Article |
