NDLI: Surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS): A new proteomic urinary test for patients with urolithiasis

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Surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS): A new proteomic urinary test for patients with urolithiasis

Content Provider	Scilit
Author	Cadieux, Peter A. Beiko, Darren T. Watterson, James D. Burton, Jeremy Howard, Jeffrey C. Knudsen, Bodo E. Gan, Bing Siang McCormick, John K. Chambers, Ann F. Denstedt, John D. Reid, Gregor
Copyright Year	2004
Description	Journal: Journal of Clinical Laboratory Analysis SELDI‐TOF‐MS is a highly sensitive protein‐analysis tool capable of detecting minute protein profile differences between biological samples. As proteins have been associated with urinary tract calculi, protein‐based urinalysis may offer insights into their diagnosis. The purpose of this study was to evaluate SELDI‐TOF‐MS as a potential method for identifying urinary biomarkers of urolithiasis. Midstream sterile urine samples were obtained from 25 male patients with a confirmed diagnosis of urolithiasis (test group) and 25 male subjects with no known history of the disease (controls). Urinary levels of oxalate, total protein, albumin, and osteopontin were determined. Protein profiles were generated using SELDI‐TOF‐MS. SELDI‐TOF‐MS profiling revealed a relationship between protein peak intensities at 67 and 24 kDa that differed between the two groups. The ratio of p67:p24 was found to be less than 1.0 in all of the control samples (mean 0.26), while 18 out of 25 (72%) of the test group samples displayed a ratio greater than 1.0 (total group mean 4.75, P<0.001). Albumin, total protein, and oxalate levels were higher in the test group than the controls. Although SELDI‐TOF‐MS is not yet in widespread use in hospital and diagnostic laboratories, this system represents a promising new method for rapidly identifying patients with urolithiasis. J. Clin. Lab. Anal. 18:170–175, 2004.
Ending Page	175
Starting Page	170
ISSN	00221295
e-ISSN	10982825
DOI	10.1002/jcla.20018
Journal	Journal of Clinical Laboratory Analysis
Issue Number	3
Volume Number	18
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2004-01-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Clinical Laboratory Analysis Medical Laboratory Technology Urinary Tract Calculi Protein Profiling
Content Type	Text
Resource Type	Article
Subject	Physiology

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