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Identification of Binding Domains of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) for its Type 1 Receptor by Photoaffinity Labelinga
| Content Provider | Scilit |
|---|---|
| Author | Cao, Yong-Jiang Kojro, Elzbieta Jasionowski, Marek Lankiewicz, Leszek Grzonka, Zbigniew Fahrenholz, Falk |
| Copyright Year | 1998 |
| Description | Journal: Annals of the New York Academy of Sciences Structure-function studies and photoaffinity labeling experiments were performed to identify residues and domains of PACAP involved in the interaction with PACAP receptors. For this purpose, a series of photoreactive analogues of PACAP(1-27) containing a photoreactive benzophenone (BP) residue in different peptide structural domains were utilized to analyze the interaction of PACAP(1-27) with pig PACAP type 1 receptors. Five PACAP derivatives were created with a photoreactive amino acid in the following peptide domains: either the disordered N-terminal or the helical C-terminal domain or a short loop region within the C-terminal helical domain of the peptide. Their receptor binding properties and efficiencies were tested on pig brain PACAP receptors. The results indicate the importance of the helical C-terminal domain of PACAP(1-27) for receptor binding affinity. Monoiodination of the photoreactive analogues did not change their binding affinities. Experiments with pig brain membranes demonstrated that the$ ^{125}$I-labeled photoreactive analogues specifically label a protein band of Mr66,000. The efficiency of photoreactive labeling differed for the various analogues. These findings suggest that $Tyr^{22}$ and $Lys^{15}$ in PACAP(1-27) are located in or close to the hormone binding site of the PACAP type 1 receptor. The results provide evidence that the α-helical C-terminal region of PACAP is directly involved in receptor binding. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1749-6632.1998.tb11166.x |
| Ending Page | 91 |
| Page Count | 10 |
| Starting Page | 82 |
| e-ISSN | 17496632 |
| DOI | 10.1111/j.1749-6632.1998.tb11166.x |
| Journal | Annals of the New York Academy of Sciences |
| Issue Number | 1 VIP, PAC |
| Volume Number | 865 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1998-12-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Annals of the New York Academy of Sciences Biochemistry and Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
